Mathematical planning of propionic acid bacteria cultivation

Igor Pavlov; Oksana Gora

doi:10.52957/27821900_2022_03_08

UDC

579.872.1 579.22

MATHEMATICAL PLANNING OF PROPIONIC ACID BACTERIA CULTIVATION Mathematical planning of propionic acid bacteria cultivation

АА Аа

Published in From Chemistry Towards Technology Step-By-Step · Pages 8–16 · Rubric: Scientific articles

DOI: https://doi.org/10.52957/27821900_2022_03_08

Received: 21.06.2022 Accepted: 12.09.2022 Published: 23.09.2022

Authors

Igor Pavlov

ORCID

Oksana Gora

Igor Pavlov

Academic degree: candidate of technical sciences

Science rang: docent

Biysk Technological Institute is a Subsidiary of Polzunov Altai State Technical University associate professor · Department of Machine and Apparatus of Chemical and Food Production Engineering Chair · Russian Federation · Biysk

ORCID

Oksana Gora

Biysk Institute of Technology, Polzunov Altai State Technical University

ABSTRACT

Based on our own research results and analysis of literature data we have proposed conditions and composition of nutrient media for the cultivation of Propionibacterium freudenreichii. As an inoculum we used propionic acid bacteria concentrate, which contains cells of selective strains of Propionibacterium freudenreichii. In the nutrient medium we kept the following concentrations constant: inoculum - 5% vol, cobalt chloride CoCl2 at a dose of 20 mg/l, and hydrolysed milk concentration - 5% vol. The contents of yeast autolysate, ascorbic acid, ammonium sulphate and lactose were varied in the composition of the nutrient medium. We cultivated the resulting mixture in a thermostat for 7 days at 30 °C and a pH of 7.0. The cultivation process was optimized by the Box-Wilson method. Using the method of mathematical planning we designed a full factor experiment FFE 2n, which allowed us to optimize the nutrient medium for propionic acid bacteria with the highest increase in bacterial biomass and accumulation of vitamin B12. We found optimal cultivation conditions for the Propionibacterium freudenreichii biomass accumulation, where the maximum biomass value at day 5 of cultivation was 60.5 g/l with high viable probiotic bacteria cell content of 12×1012 CFU/cm3 and maximum vitamin B12 accumulation at day 5 of cultivation equal to 108.1 µg/ml.

KEYWORDS

propionic acid bacteria, Propionibacterium freudenreichii, cultivation, process optimisation, nutrient medium, Box-Wilson method, vitamin B12, full-factor experiment

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Editor in chief

Dmitry Berezin

Deputy Editor in Chief

Igor Abramov

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Zhanna Chirkova

chirkovazhv@ystu.ru

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Pavlov I. N., Gora O. N. Mathematical planning of propionic acid bacteria cultivation // From Chemistry Towards Technology Step-By-Step. 2022. no. 3. pp. 8-16. DOI: https://doi.org/10.52957/27821900_2022_03_08. (Date of access 07.09.2026).

MLA

Igor Pavlov, Gora O. N.. "Mathematical planning of propionic acid bacteria cultivation." From Chemistry Towards Technology Step-By-Step 3.3 (2022): 8-16. DOI: https://doi.org/10.52957/27821900_2022_03_08.

APA

Pavlov I. N., Gora O. N. (2022). Mathematical planning of propionic acid bacteria cultivation. From Chemistry Towards Technology Step-By-Step, 3(3), 8-16. https://doi.org/10.52957/27821900_2022_03_08

Chicago

Igor Pavlov, Gora O. N.. "Mathematical planning of propionic acid bacteria cultivation." From Chemistry Towards Technology Step-By-Step 3, no. 3 (2022): 8-16. https://doi.org/10.52957/27821900_2022_03_08.

WIKI

{{cite journal | author = Pavlov I. N., Gora O. N. | title = Mathematical planning of propionic acid bacteria cultivation | journal = From Chemistry Towards Technology Step-By-Step | year = 2022 | volume = 3 | issue = 3 | pages = 8-16 | doi = 10.52957/27821900_2022_03_08 }}

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MATHEMATICAL PLANNING OF PROPIONIC ACID BACTERIA CULTIVATION Mathematical planning of propionic acid bacteria cultivation

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