employee
Biysk, Barkaul, Russian Federation
Biysk, Barkaul, Russian Federation
UDK 579.22 Биохимия и физиология микроорганизмов
Based on our own research results and analysis of literature data we have proposed conditions and composition of nutrient media for the cultivation of Propionibacterium freudenreichii. As an inoculum we used propionic acid bacteria concentrate, which contains cells of selective strains of Propionibacterium freudenreichii. In the nutrient medium we kept the following concentrations constant: inoculum - 5% vol, cobalt chloride CoCl2 at a dose of 20 mg/l, and hydrolysed milk concentration - 5% vol. The contents of yeast autolysate, ascorbic acid, ammonium sulphate and lactose were varied in the composition of the nutrient medium. We cultivated the resulting mixture in a thermostat for 7 days at 30 °C and a pH of 7.0. The cultivation process was optimized by the Box-Wilson method. Using the method of mathematical planning we designed a full factor experiment FFE 2n, which allowed us to optimize the nutrient medium for propionic acid bacteria with the highest increase in bacterial biomass and accumulation of vitamin B12. We found optimal cultivation conditions for the Propionibacterium freudenreichii biomass accumulation, where the maximum biomass value at day 5 of cultivation was 60.5 g/l with high viable probiotic bacteria cell content of 12×1012 CFU/cm3 and maximum vitamin B12 accumulation at day 5 of cultivation equal to 108.1 µg/ml.
propionic acid bacteria, Propionibacterium freudenreichii, cultivation, process optimisation, nutrient medium, Box-Wilson method, vitamin B12, full-factor experiment
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