Красноярск, Красноярский край, Россия
The paper analyses the experimental results of microbiological utilisation of mononitrotoluene and phenol at concentrations of 20, 50 and 70 mg/l by strains of Trichoderma fungi. According to the research, all the strains under study are able to grow in the presence of mononitrotoluene as the only source of nitrogen nutrition and phenol as the only source of carbon and energy. However, the strains have different sensitivity to changes in their concentration in the medium. Indeed, the strains under study are able to degrade mononitrotoluene and phenol in liquid medium. The maximum efficiency of mononitrotoluene biodegradation was 66% using Trichoderma koningii strain ‘TSL-06’, and that of phenol was 95% using Trichoderma harzianum strain ‘M99/5’. Based on the results, we selected perspective strains for biodegradation of mononitrotoluene and phenol by surface and deep cultivation methods, as well as for the development of a biopreparation based on fungi of the genus Trichoderma in an immersion biotechnological system.
biodegradation, aromatic nitro compounds, phenols, Trichoderma fungi, wastewater, utilisation, culturing
1. Zbarsky V. L., Zhilin V. F. Toluene and its nitro derivatives. 2nd ed., rev. and substantially supplemented. - M. : URSS, 2019. 344 p. (in Russian).
2. Sugak N. Yu. Methods of destruction of explosive materials. Chemical decomposition of explosives in acid and alkaline media: textbook. Biysk: Izd-vo Alt. gos. tekh. un-ta. 2007. 63 p. (in Russian).
3. Putis S. M., Ilyushin M. A. Chemical technology of energy-rich substances. Nitration of aromatic hydrocarbons : textbook for universities. St. Petersburg : Lan. 2022. 92 p. (in Russian).
4. Korobov V. V., Zharikova N. V., Anisimova L. G., Yasakov T. R., Zhurenko, E. Y., Kusova I. V., Mrkusheva T. V. Bacillus subtilis B-1742D – destruktor fenola i 2,4-dihlorfenola Bacillus subtilis B-1742D - a destructor of phenol and 2,4-dichlorophenol // Izvestiya ufimskogo nauchnogo centra RAN. Biologiya, biohimiya i genetika [Izvestiya Ufa Scientific Centre of RAS. Biology, biochemistry and genetics]. 2011. № 3-4. Pp. 52-56. Available at: http://journal.ufaras.ru/wp-content/uploads/2022/02/izvestiya_3-4_2011.pdf (accessed 08.09.2024) (in Russian).
5. Maukonen J. Methodologies for the characterization of microbes in industrial environments: a review // J. Industrial Microbiology and Biotechnology. 2003. V. 52, 1. 6. P. 327–356.
6. Nosulich V. E., Bykov A. G., Kuvichkina T. N., Makarenko A. A., Reshetilov A. N. Mikroorganizmy s fenooldegradiruyushchimi svojstvami iz ochistnyh sooruzhenij [Microorganisms with phenoldegrading properties from sewage treatment plants] // Izvestiya TulGU. Estestvennye nauki [Proceedings of TulSU. Natural Sciences]. 2018. Vol. 3, Pp. 98-103. Available at: https://tidings.tsu.tula.ru/tidings/index.php?id=search_a (accessed 08.09.2024) (in Russian).
7. Khasaeva F. M. Aspekty mikrobnyh tekhnologij primenitel'no k ohrane ob"ektov okruzhayushchej sredy. V kn: Social'no-ekonomicheskie i ekologicheskie aspekty razvitiya Prikaspijskogo regiona [Aspects of microbial technologies in relation to the protection of environmental objects. In: Socio-economic and environmental aspects of the development of the Caspian region] // Materialy Mezhdunarodnoj nauchno-prakticheskoj konferencii [Materials of the International Scientific and Practical Conference]. Nalchik. 2019. Pp. 582-585. (In Russian).
8. Korobov V. V., Zhurenko E. Yu., Zharikova N. V., Yasakov T. R. Ochistka fenolsoderzhashchih stochnyh vod s primeneniem shtamma Serratia marcescens MT9 [Treatment of phenol-containing wastewater with the use of strain Serratia marcescens MT9] // Ekologiya i promyshlennost' Rossii [Ecology and Industry of Russia]. 2022. Vol. 26. No. 2. Pp. 39-43. DOI: https://doi.org/10.18412/1816-0395-2022-2-39-43 (in Russian).
9. Khilyas V. I. Safiullina L. F., Rodionov A. A., Ziganshin A. M. Biodegradaciya 2,4,6 – trinitrotoluola gemiaskomicetnymi drozhzhami v usloviyah nepreryvnogo rezhima kul'tivirovaniya [Biodegradation of 2,4,6 - trinitrotoluene by hemiascomycetous yeasts under conditions of continuous cultivation] // Uchenye zapiski Kazanskogo universiteta. Estestestvennye nauki [Scientific Papers of Kazan University. Natural Sciences]. 2010. Vol. 152, Book 4. 179-189 p. Available at: https://kpfu.ru/portal/docs/F_1622022005/152_4_est_16.pdf (accessed 01.09.2024).
10. Kulkarni M., Chaudhari A. Microbial remediation of nitro-aromatic compounds: An overview // J. Environmental Management. 2007. Vol.85 (2). P. 496-512.
11. Marvin-Sikkema F. D., de Bont J. A. M. Degradation of nitroaromatic compounds by microorganisms // Applied Microbiology and Biotechnol. 1994. Vol. 42. P. 499–507.
12. Zhang M., Liu G.-h., Song K. [et al.] Biological treatment of 2,4,6-trinitrotoluene (TNT) red water by immobilized anaerobic–aerobic microbial filters // Chem. Eng. J. 2015. Vol. 259. P. 876–884.
13. Samarskaya Y. V., Abdrakhmanova A. S. Primenenie konsorciuma bakterij i gribov pri ochistke stochnyh vod neftepererabatyvayushchego kompleksa [Application of a consortium of bacteria and fungi in wastewater treatment of oil refining complex] // VII Mezhdunarodnaya nauchno-prakticheskaya konferenciya «Bezopasnost' gorodskoj sredy» [VII International Scientific and Practical Conference ‘Safety of Urban Environment’]. Omsk. 2019. Pp. 36-40. (In Russian).
14. Kosobrinova S. A., Yanukevich A. A., Shulkin L. L. Bioremediaciya zagryaznennyh vodnyh sred, problematika i sostoyanie voprosa [Bioremediation of polluted aquatic environments, problematics and state of the issue] // Aktual'nye problemy nauki i tekhniki [Actual problems of science and technology]. 2020. Pp. 16-22. (In Russian).
15. Zharikova N. V., Galimzyanova N. F., Zhurenko E. Yu., Yasakov T. R., Korobov V. V., Sagitova A. I., Markusheva T. V. Analiz vzaimootnoshenij bakterial'nyh shtammov-destruktorov ksenobiotikov i mikromicetov roda Trichoderma [Analysis of the relationship between bacterial strains of xenobiotic degraders and microbial strains of the genus Trichoderma] // Izvestiya ufimskogo nauchnogo centra RAN. Biologiya, biohimiya i genetika [Proceedings of the Ufa Scientific Centre of the Russian Academy of Sciences. Biology, biochemistry and genetics]. 2014. No. 1. Pp. 72-75. Available at: http://journal.ufaras.ru/wp-content/uploads/2022/01/izvestiya_1_2014.pdf (accessed 08.08.2024) (in Russian).
16. Solyanikova I. P., Baskunov B. P., Baboshin M. A., Saralov A. I., Golovleva L. A. Detoksikaciya bakteriyami trinitrotoluola v vysokih koncentraciyah [Detoxification by bacteria of trinitrotoluene in high concentrations] // Prikladnaya biohimiya i mikrobiologiya [Applied Biochemistry and Microbiology]. 2012. Vol. 48. No. 1. pp. 27-34. Available at: https://www.fbras.ru/wp-content/uploads/2017/07/48_n1_annotation.pdf (accessed 08.08.2024) (in Russian).
17. Bolshova T. A., Brykina G.D., Garmash A.V., et al. Fundamentals of analytical chemistry: a textbook for universities, in 2 books, 2nd ed. - M.: Vysh. shk., 2002 (in Russian).
18. Sadykova V. S. Ecology of fungi Trichoderma (Pers: Fr) of the Yenisei River basin, their biological properties and practical application: abstract of doctoral thesis of biology. 03.02.12. M. 2012. 46 p. (In Russian).
19. Pérez–Guerra, N. A. Torrado-Agrasar, C. López-Macias and L. Pastrana. Main characteristics and applications of solid substrate fermentation // Electronic Journal of Environmental, Agricultural and Food Chemistry. 2003. Vol. 2. No 3. P. 343–350.
20. Verma M. Satinder K. Brar, R. D. Tyagi, R. Y. Surampalli, J. R. Val ́ero. Antagonistic fungi, Trichoderma spp.: Panoply of biological control // Biochemical Engineering Journal. 2007. Vol. 37. P. 1–20.
